Cell culture substrate "diXCOAT"


Collaborating with the Tokyo University Industrial Technology center, KISCO developed original cell culture substrates based on diX coating technology.


  • No animal testing, chemically (polymer) coated cell culture substrate
  • Exhibiting cell adhesion as strong as collagen
  • Smooth coating with no dispersion

diX coating features
  • Regular coating with no pinholes while it is difficult to achieve in liquid coating
  • It is able to coat any object of shapes and sizes
  • Anti-chemical resistant (acid resistant, alkaline resistant, organic solvent resistant) derived from polymer

Primary use of diXCOAT

  • Cell culture dish, bottle and bag
  • Special material, carrier of special material, micro channel, etc.
  • Biochip, biosensor (setting of DNA/enzyme/protein)

Culturing example using diXCOAT

PC12 (Rat adrenal medulla derived from pheochromocytoma)

Sowing number: 1.02×104 cells/well (24well)
Culture medium: RPMI1640 + 10% Horse Serum, 5% FBS
Culture medium differentiation: RPMI1640 + 0.1% Horse Serum, 0.05% FBS, NGF
Rat Primary Hepatocytes

Sowing number 2.0×105 cells/well (24well)
Culture medium: Williams’ Medium E + 10% FBS, 1% PSA, 10-7M Insulin, 10-6M DEX, 50 ng/ml EGF
HUVEC (Human Umbilical Vein Endothelial)

Sowing number: 8.0×104 cells/well (24well)
Culture medium: EGM-2
MCF7 (Michigan Cancer Foundation-7)

Sowing number: 8.0×104 cells/well (24well)
Culture medium: DMEM + 10% FBS, 1% PSA, 1% NEAA
HEK293(Human Embryonic Kidney 293)

Sowing number: 8.0×104 cells/well (24well)
Culture medium: DMEM + 10% FBS, 1% PSA, 1% NEAA
Other cells in valuation process
  • Mouse iPS [(Induced Pluripotent Stem) cell (differentiation promoter, maintaining undifferentiated state)]
  • Human iPS/ES (Embryonic Stem) cell
  • Caco-2 (Heterogeneous Human Epithelial Colorectal Adenocarcinoma)
  • MEF (Mouse Embryonic Fibroblast) cell

11-2, Nihonbashi Honcho 4-chome, Chuo-ku, Tokyo, 103-8410
2nd Sales Division
Life Science Company
Biotechnology Team